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light sheet microscope  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec light sheet microscope
    Light Sheet Microscope, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/light+sheet+microscope/CD258+(LIGHT)+Antibody%2C+anti-human%2C+REAfinity/bio_rxiv__64898__2025__12__23__696229-279-6-10
    Average 93 stars, based on 1 article reviews
    light sheet microscope - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Early adversity potentiates prefrontal–amygdala communication through CRH + glutamatergic neurons
    Article Snippet: .. Brain samples were imaged using a light-sheet microscope (Ultramicroscope II, LaVision Biotec) outfitted with a sCMOS camera (Andor Neo) and a 2x/0,5 NA objective lens (MVAPLAPO 2x) with a 6mm working distance dipping cap. .. Brain samples were imaged using a light-sheet microscope (Ultramicroscope II, LaVision Biotec) outfitted with a sCMOS camera (Andor Neo) and a 2x/0,5 NA objective lens (MVAPLAPO 2x) with a 6mm working distance dipping cap.

    Article Title: Neuronal substance-P drives metastasis via an extracellular RNA/TLR7 axis
    Article Snippet: .. Optically cleared tissues were imaged on a light sheet microscope (LaVision BioTec) with a 4x/0.3 LVMI-Fluar objective with 5.6–6 mm WD. .. Images were imported into Imaris and rendered in 3D.

    Article Title: Transient agonism of the sonic hedgehog pathway triggers a permanent transition of skin appendage fate in the chicken embryo.
    Article Snippet: .. Imaging was undertaken using a light sheet microscope (Ultramicroscope 2 Blaze, Miltenyi Biotec). .. LSFM data were processed using Fiji (58), before visualization with Imaris (Oxford Instruments, UK) (Fig. 3D).

    Article Title: In Vivo Tracking and 3D Mapping of Cell Death in Regeneration and Cancer Using Trypan Blue.
    Article Snippet: The cleared liver lobes were then transferred to 100% benzyl ether (Sigma 108014) for imaging and long-term storage. .. Cleared liver lobes were imaged with a light sheet microscope (Ultramicroscope II, Lavision Biotec, Bielefeld, Germany) and the ImSpector 347 software (Abberior, GöttingenGermany). .. All images were acquired using a 2× objective lens with an additional 0.8× zoom body, an NA of 0.6 (XLPLN10XSVMP, Olympus Corporation, Tokyo, Japan), and a sCMOS camera (Hamamatsu ORCA-Flash4.0, Shizuoka, Japan).

    Article Title: Stress-activated brain-gut circuits disrupt intestinal barrier integrity and social behaviour
    Article Snippet: Samples were again washed 4 times over the following 48/24 h. For tissue clearing, samples were dehydrated in methanol, then incubated in 66% dichloromethane + 33% methanol for 3/1 h, 100% dichloromethane for 15 min twice, and nally in dibenzyl ether (108014, Sigma-Aldrich) until imaging. .. Images were acquired using a LaVision light sheet microscope (LaVision BioTec, Bielefeld, Germany) with zoom body, using dynamic focus and a step size of 3 – 5 μm. ..

    Article Title: Forebrain Eml1 depletion reveals early centrosomal dysfunction causing subcortical heterotopia.
    Article Snippet: Finally, samples were incubated in dibenzyl ether (DBE, 108014-1KG; Sigma-Aldrich) overnight. .. For 3D imaging, cleared samples were imaged on a light sheet microscope (LaVision Biotec) equipped with a sCMOS camera (Andor Neo). ..

    Article Title: Stress-activated brain-gut circuits disrupt intestinal barrier integrity and social behaviour
    Article Snippet: Samples were again washed 4 times over the following 48/24 h. For tissue clearing, samples were dehydrated in methanol, then incubated in 66% dichloromethane + 33% methanol for 3/1 h, 100% dichloromethane for 15 min twice, and finally in dibenzyl ether (108014, Sigma-Aldrich) until imaging. .. Images were acquired using a LaVision light sheet microscope (LaVision BioTec, Bielefeld, Germany) with zoom body, using dynamic focus and a step size of 3 – 5 μm. ..

    Article Title: Neuronal substance-P drives breast cancer growth and metastasis via an extracellular RNA-TLR7 axis
    Article Snippet: Samples were washed in dicholoromethane overnight and cleared in dibenzyl ether (Sigma-Aldrich, 33630). .. Optically cleared tissues were imaged on a light sheet microscope (LaVision BioTec) with a 4x/0.3 LVMI-Fluar objective with 5.6-6 mm WD. .. Images were imported into Imaris and rendered in 3D.

    Imaging:

    Article Title: Transient agonism of the sonic hedgehog pathway triggers a permanent transition of skin appendage fate in the chicken embryo.
    Article Snippet: .. Imaging was undertaken using a light sheet microscope (Ultramicroscope 2 Blaze, Miltenyi Biotec). .. LSFM data were processed using Fiji (58), before visualization with Imaris (Oxford Instruments, UK) (Fig. 3D).

    Software:

    Article Title: In Vivo Tracking and 3D Mapping of Cell Death in Regeneration and Cancer Using Trypan Blue.
    Article Snippet: The cleared liver lobes were then transferred to 100% benzyl ether (Sigma 108014) for imaging and long-term storage. .. Cleared liver lobes were imaged with a light sheet microscope (Ultramicroscope II, Lavision Biotec, Bielefeld, Germany) and the ImSpector 347 software (Abberior, GöttingenGermany). .. All images were acquired using a 2× objective lens with an additional 0.8× zoom body, an NA of 0.6 (XLPLN10XSVMP, Olympus Corporation, Tokyo, Japan), and a sCMOS camera (Hamamatsu ORCA-Flash4.0, Shizuoka, Japan).

    3D Imaging:

    Article Title: Forebrain Eml1 depletion reveals early centrosomal dysfunction causing subcortical heterotopia.
    Article Snippet: Finally, samples were incubated in dibenzyl ether (DBE, 108014-1KG; Sigma-Aldrich) overnight. .. For 3D imaging, cleared samples were imaged on a light sheet microscope (LaVision Biotec) equipped with a sCMOS camera (Andor Neo). ..



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    (A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence <t>microscopy.</t> Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.
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    (A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence <t>microscopy.</t> Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.
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    (A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence <t>microscopy.</t> Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.
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    Olympus alpha3 system
    (A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence <t>microscopy.</t> Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.
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    Image Search Results


    (A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence microscopy. Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.

    Journal: bioRxiv

    Article Title: Most early-born subplate neurons persist as Layer 6b neurons in the adult mouse neocortex

    doi: 10.1101/2025.11.20.689634

    Figure Lengend Snippet: (A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence microscopy. Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.

    Article Snippet: Cleared whole brain tissues were imaged by Light sheet microscopy (Olympus, MVX10-LS), and the z-axis interval was 4 μm in the P0 sample and 9 μm in the 8 weeks.

    Techniques: Immunohistochemical staining, Expressing, Fluorescence, Microscopy